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rat anti mouse monoclonal f4 80  (Bio-Rad)


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    Structured Review

    Bio-Rad rat anti mouse monoclonal f4 80
    Rat Anti Mouse Monoclonal F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 5920 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antibody/Rat+anti+Mouse+F4%2F80/10__22203_slash_ecm__v033a11-129-8-16
    Average 96 stars, based on 5920 article reviews
    rat anti mouse monoclonal f4 80 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Mitochondrial dysfunction fuels drug resistance in adult T-cell acute lymphoblastic leukemia.
    Article Snippet: .. The next day, the membranes were incubated with horseradish peroxidase-conjugated secondary rabbit or mouse antibody (Bio-RAD, Cat#170–6515, Cat170-6516) at RT for 1 h. After adding ECL substrates (Bio-RAD, Cat#170– 5061), the blots were then visualized on LAS-4000 (FUJIFILM) or Vilber Chemiluminescence system (Vilber). ..

    Article Title: Mitochondrial dysfunction fuels drug resistance in adult T-cell acute lymphoblastic leukemia
    Article Snippet: .. The next day, the membranes were incubated with horseradish peroxidase-conjugated secondary rabbit or mouse antibody (Bio-RAD, Cat#170–6515, Cat170-6516) at RT for 1 h. After adding ECL substrates (Bio-RAD, Cat#170–5061), the blots were then visualized on LAS-4000 (FUJIFILM) or Vilber Chemiluminescence system (Vilber). ..

    Article Title: The β 2 -adrenergic biased agonist nebivolol inhibits the development of Th17 and the response of memory Th17 cells in an NF-κB-dependent manner
    Article Snippet: Antibodies were against human phospho-ser529 NF-κB p65 (1/500, clone A21012B, BioLegend, San Diego, USA), NF-κB p65 antibody (1/1,000, clone 14G10A21, BioLegend, San Diego, USA), phospho-Ser133 CREB1 (1/700, rabbit polyclonal, Cusabio, Cedarlane), phospho-Ser412-β-arrestin 1 (1/1,000, clone mAb #2416, Cell Signaling Technology, Danvers, USA), β 2 -AR (1/500, clone 4A6C9, Novusbio, Cedarlane), GAPDH (1/15,000, 10B4E3, MA000071M1m, Cusabio, Cedarlane), and α‐tubulin (1/1,000, Santa Cruz Biotechnology Inc., USA). .. Subsequently, membranes were repeatedly washed with TBST and incubated for 2 h with the appropriate HRP-conjugated secondary mouse antibody (1/1,500, Bio-Rad Laboratories) or secondary goat anti‐rabbit IgG HRP (1/5,000, Cusabio, Cedarlane) in 5% skim milk. .. Immunoreactivity was detected using the ECL prime reagent (GE Healthcare, Chicago, USA), and then the chemiluminescence signal was recorded in the Image Lab (Bio-Rad Laboratories).

    Article Title: Slc7a8 Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs
    Article Snippet: Heat-induced epitope retrieval was performed in citrate buffer pH 6,1 using the Dako Target Retrieval Solution S1699 (Dako, Carpinteria, CA, USA) and a 2100 Retriever Unit (Electron Microscopy Sciences, Washington, PA, USA). .. The sections were rinsed in PBS/Tween buffer and treated with 5% Normal Goat Serum (Dako X0907) for 30 min, after which they were incubated overnight at 4 °C with a 1:25 dilution of F4/80 monoclonal rat anti mouse antibody BM8 (#14-4801-82) (Thermo Fisher Scientific, Bedford, MA, USA) or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories, Irvine, CA, USA). .. The sections were rinsed in PBS/Tween buffer before being incubated for 60 min in 1:200 goat anti rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (#31470) (Invitrogen, Bedford, MA, USA).

    Article Title: Anti-TTR Nanobodies Allow the Identification of TTR Neuritogenic Epitope Associated with TTR-Megalin Neurotrophic Activities.
    Article Snippet: edition.. The authors acknowledge the support of the B2Tech i3S scientific Platform.. Competing interests: The authors of the manuscript have the following potential competing interest: IC, HS, PV, AW and CS are or were employees of ABLYNX at the time of work development.

    Article Title: The β 2 -adrenergic biased agonist nebivolol inhibits the development of Th17 and the response of memory Th17 cells in an NF-κB-dependent manner.
    Article Snippet: Antibodies were against human phospho-ser529 NF-kB p65 (1/500, clone A21012B, BioLegend, San Diego, USA), NFkB p65 antibody (1/1,000, clone 14G10A21, BioLegend, San Diego, frontiersin.org USA), phospho-Ser133 CREB1 (1/700, rabbit polyclonal, Cusabio, Cedarlane), phospho-Ser412-b-arrestin 1 (1/1,000, clone mAb #2416, Cell Signaling Technology, Danvers, USA), b2-AR (1/500, clone 4A6C9, Novusbio, Cedarlane), GAPDH (1/15,000, 10B4E3, MA000071M1m, Cusabio, Cedarlane), and a‐tubulin (1/1,000, Santa Cruz Biotechnology Inc., USA). .. Subsequently, membranes were repeatedly washed with TBST and incubated for 2 h with the appropriate HRP-conjugated secondary mouse antibody (1/1,500, Bio-Rad Laboratories) or secondary goat anti‐rabbit IgG HRP (1/5,000, Cusabio, Cedarlane) in 5% skim milk. .. Immunoreactivity was detected using the ECL prime reagent (GE Healthcare, Chicago, USA), and then the chemiluminescence signal was recorded in the Image Lab (Bio-Rad Laboratories).

    Purification:

    Article Title: The Escherichia coli RnlA–RnlB toxin–antitoxin complex: production, characterization and crystallization
    Article Snippet: .. The progress of the purification was analysed by SDS–PAGE (Laemmli, 1970) and a Western blot (Towbin et al., 1979) with a mouse antibody recognizing the His tag (Bio-Rad, AbD Serotec). ..

    Western Blot:

    Article Title: The Escherichia coli RnlA–RnlB toxin–antitoxin complex: production, characterization and crystallization
    Article Snippet: .. The progress of the purification was analysed by SDS–PAGE (Laemmli, 1970) and a Western blot (Towbin et al., 1979) with a mouse antibody recognizing the His tag (Bio-Rad, AbD Serotec). ..

    Staining:

    Article Title: Slc7a8 deletion is protective against diet-induced obesity and attenuates lipid accumulation in multiple organs
    Article Snippet: .. Briefly, tissue sections were stained with F4/80 rat anti mouse antibody clone A3-1 ( Bio-Rad Laboratories , Sandton , Johannesburg , South Africa ). ..

    Membrane:

    Article Title: Anti-TTR Nanobodies Allow the Identification of TTR Neuritogenic Epitope Associated with TTR-Megalin Neurotrophic Activities.
    Article Snippet: edition.. The authors acknowledge the support of the B2Tech i3S scientific Platform.. Competing interests: The authors of the manuscript have the following potential competing interest: IC, HS, PV, AW and CS are or were employees of ABLYNX at the time of work development.



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    Image Search Results


    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

    Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression

    In vivo therapeutic efficacy of advanced BAITs in a postoperative tumor model. (A) Schematic of in vivo experimental design. LLC tumors were surgically resected, and mice were re-challenged with LLC cells. Mice then received BAIT treatments. (B) Photographs of tumors and tumor weights at day 16 for each group (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗ is P < 0.001 by one-way ANOVA with Bonferroni post-hoc test). (C) Individual tumor growth curves over time (n = 5; ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (D) H&E-stained tumor sections. (E) TUNEL staining of tumor sections (brown, apoptotic cells). Black arrowheads mark TUNEL + areas. (F) Quantification of TUNEL + apoptotic cells (n = 5; ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (G) Representative immunofluorescence for Ki67 (green) in tumor tissues (nuclei in blue). The Apo-BAIT group shows greatly reduced Ki67 + proliferating cells. (H) Quantification of Ki67 + cell density (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: In vivo therapeutic efficacy of advanced BAITs in a postoperative tumor model. (A) Schematic of in vivo experimental design. LLC tumors were surgically resected, and mice were re-challenged with LLC cells. Mice then received BAIT treatments. (B) Photographs of tumors and tumor weights at day 16 for each group (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗ is P < 0.001 by one-way ANOVA with Bonferroni post-hoc test). (C) Individual tumor growth curves over time (n = 5; ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (D) H&E-stained tumor sections. (E) TUNEL staining of tumor sections (brown, apoptotic cells). Black arrowheads mark TUNEL + areas. (F) Quantification of TUNEL + apoptotic cells (n = 5; ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (G) Representative immunofluorescence for Ki67 (green) in tumor tissues (nuclei in blue). The Apo-BAIT group shows greatly reduced Ki67 + proliferating cells. (H) Quantification of Ki67 + cell density (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). Data are presented as mean ± SD.

    Article Snippet: Anti-Ki67 mouse mAb (Cat# GB121141-100), anti-CD3 mouse mAb (Cat# GB15014-100), FITC-conjugated goat anti-mouse IgG (H + L) (Cat# GB22301), and Cy5-conjugated goat anti-mouse IgG (H + L) (Cat# GB27301) for immunofluorescence assays, and DAB (SA-HRP) TUNEL apoptosis detection kit were supplied by Servicebio (Wuhan, China).

    Techniques: In Vivo, Drug discovery, Staining, TUNEL Assay, Immunofluorescence